Erythrina Cristagalli Lectin (ECL, ECA), Fluorescein
Product Sizes
5mg
£253.00
FL-1141-5MG
About this Product
- SKU:
- FL-1141
- Additional Names:
- Lectins & Glycobiology Reagents [Lectins ], Coral Tree , Galactose (beta 4) N-acetylglucosamine
- Application:
- Immunofluorescence
- Conjugate:
- FITC
- Extra Details:
- The carbohydrate structure to which ECL binds is frequently found in membrane and serum glycoproteins of mammalian origin. Sialic acid substitution on this structure appears to prevent the lectin from binding. This specificity offers an opportunity to utilize agarose bound ECL to isolate or fractionate mammalian glycoproteins. This lectin has been reported to be useful for the isolation of human natural killer (NK) cells using a negative selection panning technique (protocol available upon request or on our website). Human NK cells appear to lack accessible surface carbohydrate structures required for binding ECL and, unlike other mononuclear cells, do not adhere to ECL-coated culture dishes. Since this procedure involves a negative selection panning technique, a high recovery of viable NK cells can be obtained. The adherent cells can also be recovered by incubation in galactose or lactose. Fluorescein labeled Erythrina cristagalli lectin has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes. The excitation maximum is at 495 nm and the emission maximum is at 515 nm.
- Shipping Conditions:
- Ambient
- Storage Conditions:
- 2-8[o]C
- Supplier:
- Vector Laboratories
- Manufacturer's Data Sheet:fluorescein-erythrina-cristagalli