Skip to content

Basket

You currently have no items in your basket.

Total (excl. vat) £0.00
View basket & checkout
Antibodies

anti-Aurora B antibody [3F11]

Product Sizes
50 ul
ARG10700-50UL
Academic Pricing
Please contact us for academic pricing.
Contact Us
About this Product
SKU:
ARG10700
Additional Names:
STK12; PPP1R48; Aurora/IPL1-related kinase 2; Serine/threonine-protein kinase 5; STK-1; IPL1; ARK-2; Aurora kinase B; Aurora-related kinase 2; AIM-1; AIK2; Serine/threonine-protein kinase 12; AIM1; STK5; ARK2; aurkb-sv1; aurkb-sv2; Serine/threonine-protein kinase aurora-B; AurB; Aurora- and IPL1-like midbody-associated protein 1; EC 2.7.11.1; Aurora 1
Application:
IHC-Frozen, Immunocytochemistry, Immunofluorescence, Western Blot
Buffer:
PBS and 50% Glycerol.
CE/IVD:
RUO
Clone:
3F11
Clonality:
Monoclonal
Concentration:
1 mg/ml
Extra Details:
This gene encodes a member of the aurora kinase subfamily of serine/threonine kinases. The genes encoding the other two members of this subfamily are located on chromosomes 19 and 20. These kinases participate in the regulation of alignment and segregation of chromosomes during mitosis and meiosis through association with microtubules. A pseudogene of this gene is located on chromosome 8. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2015]
Host:
Mouse
Isotype:
IgG2a
Purification:
Affinity Purified
Reactivities:
Bovine, Horse, Human, Mouse, Rat
Shipping Conditions:
Blue Ice
Specificity:
This antibody was made against Aurora B protein and was shown not to bind both Aurora A and C.
Storage Conditions:
-20[o]C
Supplier:
Arigo Biolaboratories
Type:
Antibody: Monoclonal Antibody
Immunocytochemistry: HeLa cell cultures were stained with ARG10700 anti-Aurora B antibody [3F11] (green).
Immunofluorescence: HeLa cells stained with ARG10700 anti-Aurora B antibody [3F11] (green) at 1:1000 dilution and costained with ARG52468 anti-Vimentin antibody (red) at 1:1000 dilution. DAPI (blue) for nuclear staining.
Western blot: Left: HeLa cells treated with 100 ng/ml nocodazole for 18 hours was stained with ARG10700 anti-Aurora B antibody [3F11]. Nocodazole is a microtubule polymerization inhibitor which induces cells to halt at the G2/M phase and also induces Aurora B expression. Right: Recombinant Human Aurora A, B and C were stained with ARG10700 anti-Aurora B antibody [3F11]. This antibody binds specifically to Aurora B.
Western blot: Cells were treated with 100 ng/ml of nocodazol, a microtubule depolymerizing agent which induces cells to halt at G2/M phase. HeLa, Dog A72, Horse NBL6 and Mouse KR158 cell lysates stained with ARG10700 anti-Aurora B antibody [3F11].