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Partnerships That Just...Click!
The advent of ‘click’ chemistry, through Copper-Catalyzed Azide Alkyne Cycloaddition (CuAAC), Ruthenium Catalysed Azide Alkyne Cycloaddition (RuAAC), Metal-free Click Chemistry and Strain Promoted Azide Alkyne Cycloaddition (SPAAC) offer researchers a tool kit to build a range of diverse molecules with ease.
For instance, where antibodies are being used to deliver a payload of a drug to a target cell, the ability to start with the same lot of reagents but assemble a variety of combinations of antibody, linker and payload consistently enables faster identification of key parameters – which antibody specificity works best, what length of PEG spacer is optimal to help with compound solubility, which drug conjugate is the most effective?
The Click Chemistry oYo-Link reagents from Alpha Thera enable site specific antibody labelling in as little as 1ug of antibody per reaction. They are available to introduce Azide, DBCO, Tetrazine or a thiol group to a specific site on an antibody. With Fluorophores and heterobifunctional linkers with a huge range of combinations of moieties on different length discrete PEG (dPEG) back bones from Vector Laboratories, researchers have the ability to investigate a wide range of possibilities but with minimal variation in manufacturing steps.