Biotinylated Proteins
See all you need to know about Biotinylated Proteins
Biotinylation refers to the process of attaching a biotin molecule to a protein or other biomolecules. Biotin is a small vitamin (also known as vitamin B7 or vitamin H) that plays a crucial role in various biochemical processes in the body. Biotinylation is commonly used in molecular biology and biochemistry for various research and practical applications. This modification can be achieved through different methods, such as chemical conjugation or enzymatic tagging.
| ELISA (Enzyme-Linked Immunosorbent Assay) Researchers can determine the presence and quantity of specific antibodies or antigens in a sample. This assay is valuable for diagnostic purposes and monitoring immune responses | FACS (Fluorescence-Activated Cell Sorting) By utilising biotinylated proteins, researchers can investigate protein-protein interactions, cell surface receptor expression, and intracellular signalling pathways. FACS enables the identification and characterisation of distinct cell populations based on their protein expression profiles. |
| Biopanning (Phage Display) Biotinylated proteins are essential for selecting phage-displayed peptides or proteins with high binding affinity to a specific target. This technique helps in discovering novel ligands or epitopes for the biotinylated protein. | SPR (Surface Plasmon Resonance) Researchers can study the kinetics of molecular interactions, such as protein-protein or protein-ligand interactions, by immobilising biotinylated proteins on a surface. SPR provides information on association and dissociation rates, binding affinities, and conformational changes. |
| BLI (Biolayer Interferometry) By using biotinylated proteins, researchers can measure real-time binding interactions between the biotinylated protein and its binding partner. BLI offers label-free and high- hroughput analysis of biomolecular interactions. | Enrichment Assays Biotinylated proteins can be utilised to enrich specific targets from complex mixtures. Researchers can use streptavidin-coated magnetic beads or other affinity matrices to isolate biotinylated proteins or interacting partners, allowing further downstream analysis and characterisation. |
Our partners at ACRO Biosystems provide an extensive selection of biotinylated proteins with remarkable attributes such as high detection sensitivity, bioreactivity, and consistency. These biotinylated proteins are designed to offer exceptional performance and reliability in various applications.
Additionally, ACRO Biosystems provides an exclusive collection of ready-to-use Avitag™ biotinylated proteins produced using the Avitag™ technology. The Avi tag, a 15-amino acid peptide, is introduced into the recombinant protein during expression vector construction. Enzymatic biotinylation occurs only on the Avi tag's lysine residue, preserving the target protein's natural binding activities. When immobilised on an avidin-coated surface, the protein orientation remains uniform, making it advantageous for common binding assays. This innovative approach offers reliable and efficient biotinylation solutions.
ACRO Biosystems utilises its proprietary HEKMax® expression platform to produce recombinant proteins, including biotinylated proteins. Employing the human HEK293 cells as the expression hosts offers a myriad of advantages over other cell types, as outlined in the table below.
Expression Systems | E.Coli | Insect Cell | Plant Cell | CHO Cell | Human Cell |
|---|---|---|---|---|---|
Folding | + | ++ | +++ | ++++ | +++++ |
Phosphorylation | N/A | ++ | +++ | ++++ | +++++ |
Proteolytic Processing | N/A | ++ | +++ | ++++ | +++++ |
Glycosylation | N/A | Poor | Poor | Non-Human Like | Human Authentic |
ACRO Biosystems Top Selling Biotinylated Proteins
Biotinylated Human PD-1 / PDCD1 Protein, Avitag[TM],His Tag (recommended for biopanning) (MALS verified)
Biotinylated Human PD-L1 / B7-H1 Protein, Avitag[TM],His Tag (MALS verified)
Biotinylated Human ACE2 / ACEH Protein, His,Avitag[TM] (MALS verified)
Biotinylated Human CD47 Protein, His,Avitag[TM]
Biotinylated Human Fc gamma RI / CD64 Protein, His,Avitag[TM] (SPR & BLI & MALS verified)
Streptavidin-coated ELISA
Streptavidin-coated ELISA plates, in conjunction with biotinylated proteins, provide a highly sensitive, specific, and versatile platform for protein detection and quantification. This robust combination is widely used in research, diagnostics, and drug discovery, facilitating a wide range of applications in various fields of biotechnology and life sciences.
Advantages of Using Streptavidin-Coated ELISA Plates
Strong and Specific Binding: The streptavidin-biotin interaction is one of the strongest and most specific non-covalent interactions known in biology. This interaction ensures a robust and stable attachment of the biotinylated protein to the streptavidin-coated plate surface.
Minimal Background Signal: Streptavidin-coated plates have very low non-specific binding, which reduces background signal in the ELISA assay. This feature enhances the sensitivity and accuracy of the assay, particularly in detecting low-abundance proteins or analytes.
Versatility: Streptavidin-coated plates can be used with a wide range of biotinylated proteins, including biotinylated antibodies, antigens, and other biomolecules. This flexibility allows for the development of diverse ELISA assays to detect various target.
| Streptavidin-Coated ELISA Plates | Biotinylated Antibodies | Biotinylated Proteins | Biotinylated DNA Aptamers |
2BScientific Limited not only prides itself on its services, but in doing so we ensure that our suppliers are something to be proud of also! That’s why we have partnered with ACRO Biosystems, Echelon Biosciences, Medix Biochemica Group, Vector Laboratories to provide our customers to ensure we can provide only the most cutting edge, reliable and accurate biotinylation tools.