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List of videos and webinars from our Suppliers

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Webinars

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Troubleshooting background in IHC and IF applications

Although the causes of non-specific background staining in immunohistochemistry (IHC) and immunofluorescence (IF) applications may differ slightly, they can obscure the detection of your specific signal. Learn about the sources of background staining and how to troubleshoot unwanted background in IHC and IF applications. 
 
Topics of discussion include: 
- Why background staining is problematic 
- How to identify sources of background 
- Strategies used to eliminate background 




Control sections for IHC & IF applications

Why to use them and what is an appropriate control?

View this webinar for a discussion on the types of controls to use, and what makes an appropriate positive and negative control. 
 
Topics of discussion include: 
- Controls to help troubleshoot background 
- Positive controls to assess assay sensitivity 
- Negative controls to identify false positive staining 

Maximizing Signal Retention in IF Applications

Low signal and loss of signal frustrate investigators as these issues are often encountered when performing immunofluorescence (IF) assays. Learn techniques and strategies that maximize signal intensity and retention in cell- and tissue-based IF. 
 
Topics of discussion include: 
- Optimizing IF workflow 
- Detection methodologies 
- Preserving the stained specimen




Counterstaining Tips and Caveats in IHC

Counterstains are often applied haphazardly at the end of an IHC procedure with little thought given to optimizing conditions for providing the best contrast with the specific antigen staining. Poor application or selection of a counterstain frequently causes a multitude of issues including color incompatibility, loss of substrate precipitate and obstruction of target antigen staining. Please join our webinar to learn about considerations when applying a counterstain and tips to avoid commonly encountered issues.

Considerations for Selecting IHC Detection Reagents

Counterstains are often applied haphazardly at the end of an IHC procedure with little thought given to optimizing conditions for providing the best contrast with the specific antigen staining. Poor application or selection of a counterstain frequently causes a multitude of issues including color incompatibility, loss of substrate precipitate and obstruction of target antigen staining. Please join our webinar to learn about considerations when applying a counterstain and tips to avoid commonly encountered issues.