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Antibodies

anti-beta III Tubulin antibody [TU-20]

Product Sizes
50 ug
ARG62683-50UG
100 ug
ARG62683-100UG
Academic Pricing
Please contact us for academic pricing.
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About this Product
SKU:
ARG62683
Additional Names:
CDCBM1; Tubulin beta-4 chain; Tubulin beta-3 chain; CFEOM3A; Tubulin beta-III; TUBB4; CDCBM; CFEOM3; FEOM3; beta-4
Application:
Flow Cytometry, IHC-Frozen, IHC-Paraffin, Immunocytochemistry, Immunofluorescence, Western Blot
Buffer:
PBS (pH 7.4) and 15 mM Sodium azide
CE/IVD:
RUO
Clone:
TU-20
Clonality:
Monoclonal
Concentration:
1 mg/ml
Extra Details:
The beta III-Tubulin isoform is present dominantly in cells of neuronal origin and it is one of the earliest markers of neuronal differentiation. It is regarded as a specific probe for the cells of neuronal origin as well as for the tumours originating from these cells. The betaIII-Tubulin is most abundant in cells of neuronal origin, but was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues.
Host:
Mouse
Isotype:
IgG1
Purity:
>95%
Purification:
Caprylic Acid Precipitated; Ammonium Sulfate Precipitated
Reactivities:
Avian, Bovine, Fish, Hamster, Human, Mouse, Non-human Primate, Porcine, Rat, Wide/Non-species Specific
Shipping Conditions:
Blue Ice
Specificity:
The clone TU-20 recognizes C-terminal peptide sequence ESESQGPK (aa 441-448) of neuron-specific human beta III Tubulin.
Storage Conditions:
-20[o]C
Supplier:
Arigo Biolaboratories
Type:
Antibody: Monoclonal Antibody
Immunohistochemistry: Paraffin-embedded Human brain tissue stained with ARG62683 anti-beta III Tubulin antibody [TU-20].
Western blot: 30 ug of 1) HeLa (negative control), 2) Mouse brain, and 3) Rat brain lysate stained with ARG62683 anti-beta III Tubulin antibody [TU-20] at 1:1000 dilution.
Immunofluorescence: Neuro2a mouse neuroblastoma cell stained with ARG62683 anti-beta III Tubulin antibody [TU-20] (green). Cell nuclei was stained with DAPI (blue).
Immunofluorescence: P-19 mouse embryonal carcinoma cells stimulated to neuronal differentiation by retinoic acid stained with ARG62683 anti-beta III Tubulin antibody [TU-20] (red). Cell nuclei was stained with DAPI (blue).
Immunofluorescence: P-19 mouse embryonal carcinoma cell line stimulated to neuronal differentiation by retinoic acid co-stained with stained with ARG62683 anti-beta III Tubulin antibody [TU-20] (red) and anti-beta-tubulin (green) Superposition of red and green colours provided yellow staining. Nuclei were stained with DNA-binding dye (blue).