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Antibodies

anti-6X His tag antibody [SQab1879]

Product Sizes
50 ul
ARG66331-50UL
100 ul
ARG66331-100UL
Academic Pricing
Please contact us for academic pricing.
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About this Product
SKU:
ARG66331
Application:
Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
Buffer:
PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
CE/IVD:
RUO
Clone:
SQab1879
Clonality:
Monoclonal
Host:
Rabbit
Isotype:
IgG
Purification:
Protein A Purified; Affinity Purified
Reactivities:
Other Species
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C
Supplier:
Arigo Biolaboratories
Type:
Antibody: Monoclonal Antibody
Flow Cytometry: 293 cells transfected with N-terminal (blue) and C-terminal (red) 6X His tagged gene. The cells were stained with ARG66331 anti-6X His tag antibody [SQab1879] at 1:1,000 dilution in 1x PBS/1% BSA for 30 min at at RT, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (black) was used as a control.
Immunofluorescence: 293 cells trasnfected with N-terminal 6X His tagged gene. Cells were fixed with 4% paraformaldehyde for 30 min at RT, permeabilized with 0.1% Triton X-100 for 10 min at RT then blocked with 10% goat serum for 30 min at RT. Cells were stained with ARG66331 anti-6X His tag antibody [SQab1879] (green) at 1:10,000 and 4°C. DAPI (blue) was used as the nuclear counter stain.
Immunofluorescence: 293 cells trasnfected with C-terminal 6X His tagged gene. Cells were fixed with 4% paraformaldehyde for 30 min at RT, permeabilized with 0.1% Triton X-100 for 10 min at RT then blocked with 10% goat serum for 30 min at RT. Cells were stained with ARG66331 anti-6X His tag antibody [SQab1879] (green) at 1:800 and 4°C. DAPI (blue) was used as the nuclear counter stain.
Immunoprecipitation: 0.05 mg of 293 whole cells lysate transfected with N-terminal 6X His tagged gene immunoprecipitated (1:50) and stained with ARG66331 anti-6X His tag antibody [SQab1879]. 1) ARG66331 IP in 293 whole cell lysate transfected with N-terminal 6X His tagged gene, 2) PBS instead of ARG66331 in 293 whole cell lysate transfected with N-terminal 6X His tagged gene, and 3) 293 whole cell lysate transfected with N-terminal 6X His tagged gene, 10 ug (input).
Immunoprecipitation: 0.2 mg of 293 whole cells lysate transfected with C-terminal 6X His tagged gene immunoprecipitated (1:50) and stained with ARG66331 anti-6X His tag antibody [SQab1879]. 1) ARG66331 IP in 293 whole cells lysate transfected with C-terminal 6X His tagged gene, 2) PBS instead of ARG66331 in 293 whole cells lysate transfected with C-terminal 6X His tagged gene, and 3) 293 whole cells lysate transfected with C-terminal 6X His tagged gene, 10 ug (input).