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Antibodies

anti-DDDDK tag antibody [SQab1880]

Product Sizes
50 ul
ARG66332-50UL
100 ul
ARG66332-100UL
Academic Pricing
Please contact us for academic pricing.
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About this Product
SKU:
ARG66332
Application:
Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
Buffer:
PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
CE/IVD:
RUO
Clone:
SQab1880
Clonality:
Monoclonal
Host:
Rabbit
Isotype:
IgG
Purification:
Protein A Purified; Affinity Purified
Reactivities:
Other Species
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C
Supplier:
Arigo Biolaboratories
Type:
Antibody: Monoclonal Antibody
Flow Cytometry: 293 cells transfected with N-terminal DYKDDDDK tagged gene. The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were stained with ARG66332 anti-DDDDK tag antibody [SQab1880] (light blue histogram) at 1:2000 dilution in 1x PBS/1% BSA for 30 min at RT, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (white histogram) was used as a control.
Immunofluorescence: 293 cells trasnfected with C-terminal DYKDDDDK tagged gene. Cells were fixed with 4% paraformaldehyde for 30 min at RT, permeabilized with 0.1% Triton X-100 for 10 min at RT then blocked with 10% goat serum for 30 min at RT. Cells were stained with DAPI (left) or ARG66332 anti-DDDDK tag antibody [SQab1880] at 1:10000 and 4°C (right).
Immunoprecipitation: 0.1 mg of 293 whole cells lysate transfected with N-terminal His tagged gene immunoprecipitated (1:50) and stained with ARG66332 anti-DDDDK tag antibody [SQab1880]. 1) PBS (Control) and 2) Primary antibody.
Western blot: Testing of ARG66332 anti-DDDDK tag antibody [SQab1880]. 3 ug of 293 cell lysate without any transfection (ARG66332 at 1:2000, left) or transfected with N-terminal DYKDDDDK tagged gene (ARG66332 at 1:10000, right).
Western blot: Testing of ARG66332 anti-DDDDK tag antibody [SQab1880]. 3 ug of 293 cell lysate without any transfection (ARG66332 at 1:2000, left) or transfected with C-terminal DYKDDDDK tagged gene (ARG66332 at 1:100000, right).