VectaFluor[TM] Duet Immunofluorescence Double Labeling Kit, DyLight[R] 488 Anti-Rabbit (green), DyLight[R] 594 Anti-Mouse (red)

Kit Contents:

• 15 ml 2.5% Normal Horse Serum (ready-to-use) for blocking
• 15 ml VectaFluor Duet Reagent [DyLight 488 Anti-Rabbit IgG and DyLight 594 Anti-Mouse IgG cocktail (rabbit-green, mouse-red)] made in horse, ready-to-use in 5% NHS.

DyLight 488:

• Excitation maximum: 493 nm
• Emission maximum: 518 nm
• Color: Green

DyLight 594:

• Excitation maximum: 592 nm
• Emission maximum: 617 nm
• Color: Red

DyLight fluorescent dyes are direct alternatives to traditional fluorophores such as fluorescein (FITC) and rhodamine. The excitation and emission spectra parallel that of other commercially available fluorescent reagents allowing for easy substitution into an existing protocol without requiring any further instrumentation or filter sets.

DyLight dyes offer a number of potential advantages including greater photostability and brighter fluorescence. DyLight dyes are completely stable over a pH range of 4-9 making them compatible with many aqueous-based buffers and diluents. The DyLight dyes can be applied as single labels or in combination with other DyLight dyes and fluorophores as part of a multiple immunofluorescent antigen staining methodology. The Dylight dyes currently offered are DyLight 488 (green), DyLight 549 (orange), DyLight 594 (red), and DyLight 649 (far red).