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Assay Kits & ELISAs

ImmPRESS[R]-AP Goat Anti-Rat IgG (Mouse Adsorbed) Polymer Detection Kit, Alkaline Phosphatase

Product Sizes
15ml
MP-5444-15ML
About this Product
SKU:
MP-5444
Additional Names:
ImmPRESS Enzyme Polymer Reagents [Detection Systems ]
Application:
Immunohistochemistry, IHC-Frozen, IHC-Paraffin, Immunocytochemistry
Conjugate:
AP
Extra Details:

The ImmPRESS polymerized reporter enzyme staining system uses novel conjugation and micropolymer chemistries to create a highly sensitive, ready-to-use, one-step detection system. This unique micropolymer of highly active enzyme is attached to our affinity purified secondary antibodies, producing reagents with outstanding sensitivity and low background. This secondary antibody can be used to stain mouse tissue because it is specifically adsorbed to remove cross-reacting antibodies of closely-related species.

Features:

  • Non-biotin
  • Enhanced accessibility to nuclear and membrane antigens
  • Ready-to-use in a convenient dropper bottle
  • Shorter assay time
  • Simplified multiple labelling
The ImmPRESS-AP Alkaline Phosphatase Polymer Reagent micropolymer technology limits steric interference and provides enhanced accessibility to the target avoiding the disadvantages of other polymer systems that use large dextrans or other macromolecules as backbones. The result is crisp, strong staining of antibody targets, especially nuclear and membrane antigens, and  greater sensitivity than conventional alkaline phosphatase antibody conjugates and other alkaline phosphatase polymer systems for immunohistochemistry and other applications. Immunostaining Protocol

The staining procedure is simple as shown in the diagram below. Following a blocking step with the diluted normal horse serum, sections are incubated with primary antibody. After a brief wash, the appropriate ImmPRESS Reagent is added to the sections and incubated for 30 minutes. Sections are again rinsed and the slides are developed with the peroxidase substrate of choice.

The ImmPRESS (15 ml) Kit will stain approximately 75-150 sections.

Host:
Goat
Shipping Conditions:
Ambient
Storage Conditions:
2-8 [o]C
Supplier:
Vector Laboratories