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IVD Cell Biology Reagents

IVD reagents for cell biology include blocking buffers such as bovine serum albumin (BSA) to prevent non-specific binding in an immunoassay; carrier proteins like gelatin and gamma globulins to stabilise antibodies or analytes; and enzyme substrates such as horseradish peroxidase (HRP) and alkaline phosphatase (AP) to facilitate detection.

Additional examples are media, additives and detachment agents for culturing live cells; lysates and purified proteins that can be employed as controls; protease inhibitors and phosphatase inhibitors to maintain protein functionality following cell lysis; animal serum for diluting antibody reagents; and cellular stains for monitoring viability.

Cell biology reagents may be purchased separately or can be key components of an IVD kit. In either scenario they must meet regulatory requirements for IVD use.

Apoptosis
FAM-FLICA Caspase-1Magic Red Cathepsin BFAM-FLICA Caspase-3/7
FAM-FLICA Poly CaspaseFLICA 660 Caspase-3/7Magic Red Caspase-3/7
SR-FLICA Poly CaspaseFAM-FLICA Caspase-9FAM FLISP FLCK Serine Protease
Autophagy
Autophagy Assay, Red
Caspases
FAM-FLICA Caspase-10FAM-FLICA Caspase-2FAM-FLICA Caspase-6
FAM-FLICA Caspase-8FAM-FLIVO In vivo Poly CaspasePyroptosis/Caspase-1
Cathepsins
Green Cathepin BMagic Red Cathepsin B
Magic Red Cathepsin KMagic Red Cathepsin L
Cellular Imaging
Col-F Collagen Binding ReagentHoechst 33342 Fluorescent Nucleic Acid StainAcridine Orange Staining Solution
Propidium Iodide StainCFSE Fluorescent Cellular StainDRAQ7 Far-Red Live-Cell Impermeant DNA Dye
7-AAD Red Fluorescent Live/Dead StainDRAQ5 Far-Red Fluorescent DNA DyeDAPI Nuclear Stain
Cytotoxicity
Basic Cytotoxicity AssayTotal Cytotoxicity & Apoptosis Assay
Green Live/Dead StainAdvanced Calcein AM Cell Viability Kit
Mitochondrial
MitoPT JC-1 AssayMitoPT TMRE AssayMitoPT TMRM Assay
Oxidative Stress
Hydrogen Peroxide Fluorescent Detection KitGlutathione Colorimetric Detection KitIntracellular GSH Assay
Intracellular Total ROS Activity AssayNitric Oxide Synthase AssayDNA Damage ELISA Kit