Echelon Transmembrane Proteins
PI4K Kinase is critical in membrane trafficking and is vital in the transport of proteins and lipids. It can have implications in a range of health related issues such as cancer, viral infection, metabolic disorders, and neurological disorders. Researchers are investigating PIK4 to understand its functions, this could lead to the development of new treatments and deeper insights into these diseases.
Phosphatidylinositol 4-kinase (PI4K) is an enzyme involved synthesis of its similarly named counterpart phosphatidylinositol 4-phosphate (PI4P). PI4P is a vital signalling molecule composed of lipids, it is additionally the precursor to phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) a phosphoinositide (minority phospholipid of the cell membrane) that is fundamental in intracellular signalling pathways, transport of vesicles and serving and a binding site for many proteins.
Echelon Biosciences understands the importance of research into the role of PI4K in cellular signalling. That’s why they provide high quality tools and reagents for the detection and quantification of PI4K including:
PI(4)P Mass ELISA Kit: The PI(4)P Mass ELISA Kit is purposely engineered for the precise detection and quantification of PI(4)P by using a competitive ELISA format. By removing the necessity for radioactivity and thin layer chromatography, it results in a safer and more efficient analytical process. Notably, the PI(4)P Mass ELISA demonstrates unparalleled specificity in directly identifying PI(4)P amidst all other phosphoinositides.
PI4-Kinase Activity Assay: A competitive ELISA assay is used to quantify PI(4)P levels, with the signal intensity inversely correlating to the amount of PI(4)P detected. This assay serves as a crucial tool for understanding cellular mechanisms reliant on PI(4)P, such as membrane trafficking and signalling pathways.
Purified Anti-PtdIns(4)P monoclonal antibody: Researchers can utilise Purified Anti-PtdIns(4)P monoclonal antibodies in a variety of applications, including ELISA, Immunofluorescence, Fluorescence Polarisation, Protein-Lipid Overlay. By selectively targeting PtdIns(4)P, researchers can investigate its role in cell signalling, membrane trafficking, and cytoskeletal organisation. Echelon ensure high specificity and reliability, making it an essential tool for studying lipid signalling pathways.
Subfamily | Isozyme | Location and Purpose | |
Type II Kinases | PI4KIIα | Cell Location | Golgi Apparatus |
Purpose | Maintain Golgi structure and trafficking | ||
Impact | Cancer and Neurodegenerative Disease | ||
PI4KIIβ | Cell Location | Endosomes | |
Purpose | Regulation of endosomal membrane dynamics | ||
Impact | Neurodegenerative Disorders | ||
Type III Kinases | PI4Kα | Cell Location | Membrane Functionality |
Purpose | Receptor Signalling and Membrane Trafficking | ||
Impact | Cancer, diabetes, and infectious diseases | ||
PI4Kβ | Cell Location | Endomembrane compartments | |
Purpose | Membrane trafficking at Golgi and endosomes | ||
Impact | Metabolic disorders, neurodegenerative diseases, and viral infections | ||
Echelon Bioscience Product Catalogue | ||
Antibody | ||
Proteins, Peptides, Small Molecules & Other Biomolecules | ||
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Assay Kits | ||
Buffers, Microspheres and Ligands | ||