IBA Lifesciences

Strep-Tag® - The leading affinity tag in recombinant protein production

Strep-Tag enables cloning, expression, detection, and purification in addition to detailed analysis of recombinant proteins. This one step purification utilising Strep-Tag II and Strep-Tactin ensures that the protein of interest is exceptionally decontaminated, even from crude cell lysates.

• Compatibility – Strep-Tag is compatible with an array of different protein classes including even fragile proteins with multiple subunits resulting in minimal experimental artifacts and broadening downstream options.
• Fully Functional Protein Yield – The physiological conditions result in the yield of fully functional proteins, making Strep-Tag a great solution for enzyme purification in addition to structural investigations, protein-protein interaction studies and even separation of living cells for re-culturing process.
• High Tolerance to Buffers – A high tolerance to buffers and additives results in a broader universal applicability. 
• Protein Purification – Highly selective binding properties leading to unparalleled protein purity (>95%)

Strep-Tactin®XT 4Flow® Starter Kit - Contains all essential reagents required for purification and detection of Strep-tag®II and Twin-Strep-tag® fusion proteins. This kit has a range of advantages including a broad pH compatibility, excellent pressure stability, outstanding target purity (>95%) and it is recommended for all protein classes, including low abundant proteins.

Strep-Tactin®XT BLI Coupling Kit - Intended for site-directed, reversible capture of Twin-Strep-tag^® fusion proteins for biomolecular interaction analysis using Bio-Layer Interferometry (BLI). This kit allows for the re-using of coated biosensors via regeneration in addition to pM affinity for Twin-Strep-Tag fusion protein allowing for specific capture from complex samples.

Protein Purification

His-Tag often exhibits several drawbacks since it can impact the natural conformation of target proteins or require harsh elution and wash conditions which in turn, effect the yield of the target protein.

Strep-Tag’s technology platform on the other hand, consists of two streptavidin variants in the form of Strep-Tactin and Strep-Tactin XT as well as two affinity tags, namely Strep-Tag II and Twin Strep-Tag which demonstrate high specificity leading to highly pure proteins. IBA additionally provides several resins either coupled with Strep-Tactin or Strep-Tactin The purification cycle caries slightly between the two streptavidin variants, but both have the same goal…

“Simple, fast, and variable protein purification procedures for highly pure proteins.”

Monoclonal Antibody Development & Production

The production of monoclonal antibodies is essential for research in novel therapeutics in addition to being utilised for a range of direct and indirect detection of antigens, as a capture molecule or as a ligand for protein purification. Using Strep-Tag for the antigen purification process results in a straightforward isolation procedure and highly pure antigen preparation suitable for a plethora of downstream processes. It can additionally be reused after Clean-in-place procedures.