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Mouse Interferon Products

1. Highly Active Mouse Interferon Protein

High quality recombinant proteins derived from bacterial or mammalian cells

  • Consistent performance and biological activity
  • 95% or greater purity
  • Endotoxin level < 1 EU/ug
  • Suitable for use in a range of applications including ELISA & CPE assays

Mouse IFN Alpha 4, mammalian Recombinant Type I interferon exhibiting early induction (Cat. No. 12115)

  • Provides a better model of native mouse alpha 4 through authentic sequencing.
  • May play a role in preparing cells to express high levels of other mouse IFN alpha subtypes upon viral infection.

Antiviral Activity Comparison of Mouse IFN Alpha A and IFN Alpha 4

Figure 1. (top) Antiviral Activity Comparison of Mouse IFN Alpha A and IFN Alpha 4 (mammalian). Mouse IFN Alpha 4 exhibits higher antiviral potency than Mouse IFN Alpha A in this standard CPE assay.

C12R Mouse IFN Alpha Decoy Receptor Viral C12R IFN antagonist (Cat. No. 12185)

  • Selectively neutralizes all mouse IFN alpha subtypes
  • No cross-reactivity with mouse IFN beta Mu Alpha 4, mammalian EC50=3.6 pg/ml Mu Alpha A, E.coli EC50=24 pg/

    C12R Neutralization of Mouse IFN Alpha Subtypes on L929 Cells.

Figure 2. (Above) C12R Neutralization of Mouse IFN Alpha Subtypes on L929 Cells.

2. Mouse Interferon Alpha (IFN-α) ELISAs

Featured: Mouse IFN Alpha All Subtype ELISA High Sensitivity (Cat. No. 42115)

  • Assay Standard Range: 2.38 – 152 pg/ml
  • Recognizes all 14 IFN alpha subtypes
  • Sensitive, low pg/ml global detection of mouse IFN-α subtypes
  • Robust matrix tolerance including serum, plasma, and tissue culture media (TCM)
  • Rapid ELISA provides total interferon alpha readout in ~ 2 hours
  • High data reproducibility with < 10% inter- and < 8% intra-assay CVs
Signal to Noise Ratios of Mouse IFN Alpha Subtypes in Mouse Serum.
Figure 3. Signal to Noise Ratios of Mouse IFN Alpha Subtypes in Mouse Serum.
Levels of endogenous mouse IFN alpha quantified in pooled plasma (A) and individual sera (B).
Figure 4. Levels of endogenous mouse IFN alpha quantified in pooled plasma (A) and individual sera (B).

IFN-α Related Products

To learn more, please contact Info@2BScientific.com or call us at +44 (0)1869 238033!

 
42120
42115
● Good   ● ● Better   ● ● ● Best
Mu-IFN-α
Mu-IFN-α All Subtype
Global IFN-α subtype measurement in serum or plasma
● ●
● ● ●
Global IFN-α subtype measurement in TCM
● ●
● ● ●
Reproducible results with < 10% inter- & intra-assay CVs
● ● ●
● ● ●


3. Mouse Interferon Beta (IFN-β) ELISAs

Featured: Mouse IFN Beta Serum ELISA High Sensitivity (Cat. No. 42410)

  • Assay Standard Range: 0.94 – 60 pg/ml
  • Accurately quantifies low levels of mouse IFN-β in multiple matrices
  • Provides > 90% spike-recovery in serum and plasma
  • High precision assay reproducibility with < 10% inter- and intra-assay CVs
  • Total assay time of ~ 2 hours
Representative Standard Curves. Competitor data obtained from published public source
Figure 5. Representative Standard Curves. Competitor data obtained from published public source (provided for comparison use only)
Comparison of ELISA Reactivity of Mammalian and E.coli-expressed Mouse IFN Beta. Sensitive detection of mammalian and E.coli expressed IFN-β.
Figure 6. Comparison of ELISA Reactivity of Mammalian and E.coli-expressed Mouse IFN Beta. Sensitive detection of mammalian and E.coli expressed IFN-β.

IFN-β Related Products

Cat. No

Description

Size

42400-1, 2

VeriKine Mouse IFN Beta ELISA Kit (TCM)

1 x 96-well plate,
5 x 96-well plate

42410-1, 2

VeriKine-HS Mouse IFN Beta Serum ELISA Kit (S, P, TCM)

1 x 96-well plate,
5 x 96-well plate

To learn more, please contact Info@2BScientific.com or call us at +44 (0)1869 238033!

 
42400
42410
● Good   ● ● Better   ● ● ● Best
Mu-IFN-β
Mu-IFN-β Serum
Accurate IFN-β measurement in serum or plasma
 
● ● ●
Accurate IFN-β measurement in tissue culture media (TCM)
● ●
● ● ●
Reproducible results with < 10% inter- & intra-assay CVs
● ●
● ● ●

4. Anti-Mouse IFN Monoclonal (MAb) & Polyclonal (PAb) Antibodies

Labeled & unlabeled antibodies for a variety of uses

  • MAbs offer high specificity in applications where specific binding is desirable with > 95% purity
  • PAbs are preferable for neutralization because they target a variety of specific epitopes of a particular antigen

Anti-Mouse IFN Beta Antibody, Clone RMMB-1 (MAb) (Cat. No. 22400)

  • Binds to mouse IFN-β (weakly neutralizes)
  • Suitable for use in Direct Binding ELISA

• Suitable for use in neutralization • Suggested for use in ELISA, Western Blot, immunoprecipitation, and immunohistochemistry

Figure 7. Direct Binding ELISA. Representative binding curve of Anti-Mouse IFN Beta antibody to recombinant Mouse IFN Beta and 1% BSA/ PBS

Anti-Mouse IFN Beta Antibody, Rabbit IgG, Protein A Purified (PAb) (Cat. No. 32401)

  • Suitable for use in neutralization
  • Suggested for use in ELISA, Western Blot, immunoprecipitation, and immunohistochemistry
    Titration against Mammalian IFN. Neutralization of 32401 Rabbit PAb stocks using mammalian-expressed Mouse IFN Beta standard.

Figure 8. Titration against Mammalian IFN. Neutralization of 32401 Rabbit PAb stocks using mammalian-expressed Mouse IFN Beta standard.