RNAConnect
RNAConnect was founded by innovators in the RNA community, for innovators in the RNA community. By providing better enzymatic tools for visualizing and manipulating RNA, we can help our community discover, create, and innovate in ways previously not possible.
Conventional RNA sequencing technologies are built on platforms that use legacy enzymes discovered decades ago. These are not ideal for modern applications that require an accurate reflection of transcriptomic diversity under real-world conditions. At RNAConnect, we focus on providing better enzymatic tools to unlock the full potential of your research and to maximize your productivity.
2BScientific Limited is proud to announce the distribution of RNAConnect’s ground-breaking UltraMarathonRT (uMRT) technology. This next-generation reverse transcriptase sets a new gold standard in the field, offering unparalleled visibility and performance for multiomics research. With 2BScientific's effective and customer centric distribution, reliable access, and plethora of advantages over competing distributors, it empowers researchers and scientists to make significant advancements in their studies quickly and efficiently.
RNAConnect is redefining reverse transcriptase technology, providing unmatched visibility and performance crucial for understanding biological functions at all levels, from patient samples to single cells. uMRT captures the natural diversity of RNA by copying each transcript end-to-end in a single pass. This unbiased approach is crucial for accurate transcriptome analysis, as it ensures that all variations within the RNA are detected. UltraMarathonRT (uMRT) generates long cDNA (up to 30kb), unwinds stable structures, is compatible with high temperature applications (42-55ºC), and boasts high processivity and fidelity. Replacing the standard reverse transcriptase with uMRT has enabled the identification of 25% more genes. This increase in detection power ensures that critical genetic information is not overlooked, providing a more comprehensive understanding of the transcriptome.
One of the standout features of uMRT is its ability to offer consistent transcript coverage from the 5' to 3' ends of RNA transcripts. This consistency effectively addresses the common problem of 3' bias, ensuring that the data obtained is reliable and comprehensive. Researchers can trust that their findings reflect the true state of the transcriptome without the distortions typically introduced by uneven coverage. Moreover, uMRT excels in handling stable RNA structures, thanks to its natural helicase activity. This ability allows it to read through complex RNA structures, which are often challenging for other reverse transcriptase. As a result, uMRT can reveal RNA structures in experiments such as SHAPE-MaP and DMS-Map, making it an ideal tool for high-throughput mapping of RNA structures.
Another significant advantage of uMRT is its consistent terminal transferase activity, which enables complete 5' to 3' end sequencing. This feature enables accurate transcriptome analysis, providing more detailed and reliable results regarding the RNA landscape. Additionally, uMRT operates optimally at ambient temperatures (20-42ºC), preserving the integrity of delicate RNA samples. This is particularly important for long, structured RNAs or very low abundant RNAs, which degrade rapidly when exposed to higher temperatures. The uMRT Template Switching Kit offers complete coverage from 5’ to 3’ ends, is suitable for both standard and challenging templates, ideal for low-input and single-cell applications, and compatible with a range of sequencing platforms.
uMRT Publication
Characterization and implementation of the MarathonRT template-switching reaction to expand the capabilities of RNA-Seq