Neosporosis cELISA Kit from VMRD

The Neospora Antibody Test Kit from VMRD is used by researchers to detect the presence of Neospora caninum antibodies in serum samples. Neospora caninum is a protozoan parasite that can infect various animals, including cows. By testing for the presence of antibodies specific to Neospora caninum in serum, veterinarians or researchers can determine whether the cow has been exposed to the parasite and has developed an immune response against it.

Neospora-associated abortions typically show no clinical signs before or after the event. Aborted foetuses often exhibit autolysis without clear lesions, and placentas are typically not retained. Abortions can occur in cows from 3 months gestation to term, with a majority (78%) happening between 4 and 6 months through foetal development. Carrier identification relies on specific antibody detection through serological tests, and the Neosporosis cELISA kit from VMRD is highly regarded for its effectiveness in this regard.

With over 60 references, the Neospora test by VMRD utilises a competitive ELISA method to identify Neospora caninum antibodies in cattle sera. The assay captures a 65 kDa immunodominant surface protein on the antigen plate using a monoclonal antibody, while another monoclonal antibody, conjugated with Horseradish Peroxidase (HRP), competes with serum antibodies for a specific epitope on p65.

Sensitivity and specificity findings demonstrate the kit's high accuracy. In a screening of 4,323 sera with unknown serologic status, only 5% fell within ±5% of the cut-off value, illustrating a clear differentiation between positive and negative sera. Additionally, the inclusion of the wash buffer from VMRD further ensures optimal performance and reliability of the assay.

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