ZytoPure® FISH Probes for Sarcomas
Zytomed Systems is proud to offer the new product line ZytoPure® FISH, a reliable and accurate tool for the detection of genetic aberrations in FFPE tissue sections, cell samples and metaphase spreads by fluorescence in situ hybridization. ZytoPure® FISH Probes display brilliant signals, low background, and an excellent signal to noise ratio. They are intended to be used in combination with the ZytoPure® FISH Accessory Kit comprising all reagents necessary for performing a FISH procedure on FFPE sections. The kit contains just 4 reagents and includes a robust and easy to follow protocol. All ZytoPure® FISH Probes as well as the ZytoPure® FISH Accessory Kit are CE/IVD-labelled.
Background information
Numerous recurrent genomic rearrangements have been described in a number of bone and soft tissue tumors in the last 15 years [1, 2]. To give just a few examples, rearrangement of the USP6 gene occurs in the majority of primary aneurysmal bone cysts [3], and also in nodular fasciitis [4]. SS18 rearrangement is highly specific for synovial sarcomas [5], and the majority of Ewing sarcomas is characterized by EWSR1 rearrangements [1]. Amplification of the MDM2 gene typically occurs in liposarcomas (but not lipomas) [6], and in certain osteosarcomas. In combination with histological and radiological findings, FISH-based detection of these rearrangements is a very important tool for the often complex differential diagnosis of these entities.
Product information
ZytoPure® FISH Probes for sarcomas
Description | Labeling | CE/IVD | Volume | Cat. No. |
|---|---|---|---|---|
MDM2/CEP12 ZytoPure® FISH Probe | green/orange | X | 20 μl | |
100 μl | ||||
USP6 Break Apart ZytoPure® FISH Probe | green/orange | X | 20 μl | |
100 μl | ||||
EWSR1 Break Apart ZytoPure® FISH Probe | orange/green | X | 20 μl | |
100 μl | ||||
SS18 Break Apart ZytoPure® FISH Probe | orange/green | X | 20 μl | |
100 μl | ||||
FUS Break Apart ZytoPure® FISH Probe | green/orange | X | 20 μl | |
100 μl |
Ancillary Reagents
Description | CE/IVD | Volume | Cat. No. |
|---|---|---|---|
ZytoPure® FISH Accessory Kit (Pepsin Solution, 4 ml; FISH Pretreatment Buffer, 500 ml; 20x FISH Wash Buffer, 2 x 50 ml; DAPI/Antifade Solution, 800 μl) | X | 1 Kit (20 tests) | |
Pepsin Solution | X | 4 ml | |
FISH Pretreatment Buffer (rtu) | X | 500 ml | |
20x FISH Wash Buffer | X | 50 ml | |
DAPI/Antifade Solution | X | 800 μl | |
Fixogum (Rubber Cement) | - | 50 g | |
125 g |
Other ZytoPure® FISH Probes
Description | Labeling | CE/IVD | Volume | Cat. No. |
|---|---|---|---|---|
ERBB2/CEP17 ZytoPure® FISH Probe | orange/green | X | 20 μl | F2C001-002 |
100 μl | F2C001-010 | |||
ALK Break Apart ZytoPure® FISH Probe | green/orange | X | 20 μl | F2C002-002 |
100 μl | F2C002-010 | |||
ROS1 Break Apart ZytoPure® FISH Probe | orange/green | X | 20 μl | F2C003-002 |
100 μl | F2C003-010 | |||
RET Break Apart ZytoPure® FISH Probe | orange/green | X | 20 μl | F2C004-002 |
100 μl | F2C004-010 | |||
MET/CEP7 ZytoPure® FISH Probe | green/orange | X | 20 μl | F2C005-002 |
100 μl | F2C005-010 |
ZytoPure ® Fluorochromes
Fluorochrome | Excitation | Emission |
|---|---|---|
PureGreen | 498 nm | 532 nm |
PureOrange | 550 nm | 576 nm |
Literature
[1] Puls F et al. Histopathol 64:461-476, 2014
[2] Vargas AC et al. Asia Pac J Clin Oncol 15:38-47, 2019
[3] Oliveira AM et al. Am J Pathol 165:1773-1780, 2004
[4] Erickson-Johnson MR et al. Lab Invest 91:1427-1433, 2011
[5] Tanas MR et al. Arch Pathol Lab Med 134:1797-1803, 2010
[6] Ware PL et al. Am J Clin Pathol 141:334-341, 2014