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Proteins and Peptides

Alpha Synuclein Monomers (Type 2)

Product Sizes
100 ug
SPR-316-100UG
2 x 100 ug
SPR-316-2X100UG
5 x 100 ug
SPR-316-5X100UG
About this Product
SKU:
SPR-316
Additional Names:
Alpha synuclein monomer, Alpha-synuclein monomer, Alpha synuclein protein monomer, Alpha synuclein monomer, Alpha-synuclein protein, Non-A beta component of AD amyloid protein, Non-A4 component of amyloid precursor protein, NACP protein, SNCA protein, NACP protein, PARK1 protein, Alpha synuclein monomers, SYN protein, Parkinson disease familial 1 Protein
Application:
Cell-based/Functional Assay, SDS-PAGE, Western Blot
CE/IVD:
RUO
Concentration:
2 mg/ml
Extra Details:
Alpha-Synuclein (SNCA) is expressed predominantly in the brain, where it is concentrated in presynaptic nerve terminals (1). Alpha-synuclein is highly expressed in the mitochondria of the olfactory bulb, hippocampus, striatum and thalamus (2). Functionally, it has been shown to significantly interact with tubulin (3), and may serve as a potential microtubule-associated protein. It has also been found to be essential for normal development of the cognitive functions; inactivation may lead to impaired spatial learning and working memory (4). SNCA fibrillar aggregates represent the major non A-beta component of Alzheimers disease amyloid plaque, and a major component of Lewy body inclusions, and Parkinson's disease. Parkinson's disease (PD) is a common neurodegenerative disorder characterized by the progressive accumulation in selected neurons of protein inclusions containing alpha-synuclein and ubiquitin (5, 6).
Immunogen:
Alpha Synuclein
Molecular Weight:
~14.46 kDa
Purity:
>95%
Purification:
Ion Exchange
Sequence:
MDVFMKGLSK AKEGVVAAAE KTKQGVAEAA GKTKEGVLYV GSKTKEGVVH GVATVAEKTK EQVTNVGGAV VTGVTAVAQK TVEGAGSIAA ATGFVKKDQL GKNEEGAPQE GILEDMPVDP DNEAYEMPSE EGYQDYEPEA
Shipping Conditions:
Dry Ice
Storage Conditions:
-70[o]C
Supplier:
StressMarq Biosciences
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Recombinant Proteins
SDS-PAGE of ~14 kDa Human Recombinant Alpha Synuclein Protein Monomer (SPR-316). Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA (2.5 ug). Lane 3: BSA (5 ug). Lane 4: Alpha Synuclein Protein Monomer (2.5 ug) (SPR-316). Lane 5: Alpha Synuclein Protein Monomer (5 ug) (SPR-316).
Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in alpha synuclein fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to alpha synuclein protein aggregation) over time when 10 uM of Type 1 alpha synuclein pre-formed fibrils (SPR-322) is combined with 100 uM of Type 1 alpha synuclein monomer (SPR-321), compared to 10 uM of Type 1 alpha synuclein pre-formed fibrils (SPR-322) combined with 100 uM Type 2 alpha synuclein monomer (SPR-316). Type 2 fibrils (SPR-317) do not seed type 2 monomers (SPR-316) (data not shown). Thioflavin T ex = 450 nm, em = 485 nm.
Type 2 monomers (SPR-316) are currently undergoing testing in a Real-Time Quaking-Induced Conversion (RT-QuIC) assay. At 10 ug/well there was discrimination between positive and negative CSF samples, and the unseeded reaction occurred later than either the LB BH or the positive CSF sample. This suggests Type 2 monomers could potentially be used as a substrate for alpha synuclein RT-QuIC. Further testing/optimization is underway. LB BH: 10% Lewy body disease; CSF +: CSF from patient with neuropathologically confirmed alpha-synucleinopathy; CSF -: CSF from patient with no evidence of alpha-synuclein deposition at postmortem. Image source: Alison Green, Graham Fairfoul
Evaluation of a-syn toxicity on primary mouse cortical neurons. Lactate dehydrogenase (LDH) is a soluble enzyme present in the cytosol that is released upon cell death. Toxicity was assessed with an LDH assay and displayed as % of vehicle control (VC). Data are presented as bar graphs and standard deviation. For statistical analysis One-way ANOVA followed by Bonferroni post-hoc test (vs VC) was used. *** p<0.001. Treatment with alpha synuclein monomers did not significantly impact LDH release. Data courtesy of QPS.
Evaluation of a-syn toxicity on primary mouse cortical neurons. Mitochondrial dehydrogenase activity reduces yellow MTT to dark blue formazan crystals, a reaction catalyzed in living cells. Cell viability was assessed with an MTT assay and displayed as % of vehicle control (VC). Data are presented as bar graphs and standard deviation. For statistical analysis One-way ANOVA followed by Bonferroni post-hoc test (vs VC) was used. ** p<0.01, *** p<0.001. Treatment with alpha synuclein monomers did not significantly impact cell viability. Data courtesy of QPS.