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Proteins and Peptides

Tau (K18) P301L Mutant Pre-formed Fibrils

Product Sizes
100 ug
SPR-330-100UG
2 x 100 ug
SPR-330-2X100UG
5 x 100 ug
SPR-330-5X100UG
About this Product
SKU:
SPR-330
Additional Names:
Tau PFFs, Tau PFF, Tau protein Pre-formed Fibrils, Tau aggregates, microtubule-associated protein Tau, MAPT, MAP, microtubule-associated protein, Paired Helical Filament-Tau, Phf-Tau, Neurofibrillary Tangle Protein, G Protein Beta1/Gamma2 Subunit-Interacting Factor 1, Isoform 4, tubulin-associated unit
Application:
Cell-based/Functional Assay, SDS-PAGE, Western Blot
CE/IVD:
RUO
Concentration:
2 mg/ml
Extra Details:
Alzheimer's Disease (AD) is the most common neurodegenerative disease, affecting 10% of seniors over the age of 65 (1). It was named after Alois Alzheimer, a German scientist who discovered tangled bundles of fibrils where neurons had once been in the brain of a deceased patient in 1907 (2). Tau (tubulin-associated unit) is normally located in the axons of neurons where it stabilizes microtubules. Tauopathies such as AD are characterized by neurofibrillary tangles containing hyperphosphorylated tau fibrils (3). There are six isoforms of tau in the adult human brain: three with four repeat units (4R) and three with three repeat units (3R) (4). K18 is a truncated form of human tau containing only the 4 microtubule binding repeats (5). P301L (PL) is a mutation where proline is replaced by leucine at codon 301 of tau, and has been linked to frontotemporal dementia (6).
Immunogen:
Tau (K18) P301L Mutant
Molecular Weight:
~15.1 kDa
Purity:
>95%
Purification:
Ion Exchange
Sequence:
SRLQTAPVPM PDLKNVKSKI GSTENLKHQP GGGKVQIINK KLDLSNVQSK CGSKDNIKHVLGGGSVQIVY KPVDLSKVTS KCGSLGNIHH KPGGGQVEVK SEKLDFKDRV QSKIGSLDNI THVPGGGNKK IETHKLTFRE
Shipping Conditions:
Dry Ice
Storage Conditions:
-70[o]C
Supplier:
StressMarq Biosciences
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Recombinant Proteins
TEM of recombinant Tau (K18), P301L mutant Pre-formed Fibrils (PFFs) at 150kx magnification. HV=80kV.
Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in tau fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to tau aggregation) over time in tau Pre-formed Fibrils (SPR-330). Tau Pre-formed Fibrils (SPR-330) seed the formation of new tau fibrils when combined with tau monomers (SPR-327). Thioflavin T ex = 450 nm, em = 485 nm. 10 uM heparin was added to each well.
SDS-PAGE of ~15 kDa Active Human Tau Protein K18 P301L Pre-formed Fibrils (SPR-330). Lane 1: MW Ladder. Lane 2: Tau Protein Pre-formed Fibrils (SPR-330).