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Proteins and Peptides

Alpha Synuclein Oligomers (Dopamine HCL Stabilized)

Product Sizes
100 ug
SPR-466-100UG
2 x 100 ug
SPR-466-2X100UG
5 x 100 ug
SPR-466-5X100UG
About this Product
SKU:
SPR-466
Additional Names:
Alpha synuclein protein, Alpha-synuclein oligomer, Alpha synuclein protein oligomer, Alpha-synuclein protein, Non-A beta component of AD amyloid protein, Non-A4 component of amyloid precursor protein, NACP protein, SNCA protein, NACP protein, PARK1 protein, Alpha synuclein oligomers, Alpha Synuclein Protein Oligomers, SYN protein, Parkinson's disease familial 1 Protein
Application:
Cell-based/Functional Assay, SDS-PAGE, Western Blot
CE/IVD:
RUO
Concentration:
2 mg/ml
Extra Details:
Alpha-Synuclein (SNCA) is expressed predominantly in the brain, where it is concentrated in presynaptic nerve terminals (1). Alpha-synuclein is highly expressed in the mitochondria of the olfactory bulb, hippocampus, striatum and thalamus (2). Functionally, it has been shown to significantly interact with tubulin (3), and may serve as a potential microtubule-associated protein. It has also been found to be essential for normal development of the cognitive functions; inactivation may lead to impaired spatial learning and working memory (4). SNCA fibrillar aggregates represent the major non A-beta component of Alzheimers disease amyloid plaque, and a major component of Lewy body inclusions, and Parkinson's disease. Parkinson's disease (PD) is a common neurodegenerative disorder characterized by the progressive accumulation in selected neurons of protein inclusions containing alpha-synuclein and ubiquitin (5, 6). Dopamine inhibits alpha synuclein fibrillogenesis leading to oligomer accumulation in vitro (7), (8) and in vivo (9), (10).
Immunogen:
Alpha Synuclein Oligomers
Molecular Weight:
~14.46kDa
Purity:
>95%
Purification:
Ion Exchange
Shipping Conditions:
Dry Ice
Storage Conditions:
-70[o]C
Supplier:
StressMarq Biosciences
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Recombinant Proteins
TEM of Human Recombinant Alpha Synuclein Oligomers (Dopamine HCL Stabilized) (SPR-466)
HPLC of Alpha Synuclein Protein Oligomers (SPR-466) ran down a size exclusion column. Wavelength: 280 nm.
Evaluation of a-syn toxicity on primary mouse cortical neurons. Lactate dehydrogenase (LDH) is a soluble enzyme present in the cytosol that is released upon cell death. Toxicity was assessed with an LDH assay and displayed as % of vehicle control (VC). Data are presented as bar graphs and standard deviation. For statistical analysis One-way ANOVA followed by Bonferroni post-hoc test (vs VC) was used. *** p<0.001. Treatment with dopamine-stabilized oligomers was accompanied by a significant increase of LDH release.
Evaluation of a-syn toxicity on primary mouse cortical neurons. Mitochondrial dehydrogenase activity, which reduces yellow MTT to dark blue formazan crystals, is a reaction that is catalyzed in living cells. Cell viability was assessed with an MTT assay and displayed as % of vehicle control (VC). Data are presented as bar graphs and standard deviation. For statistical analysis One-way ANOVA followed by Bonferroni post-hoc test (vs VC) was used. ** p<0.01, *** p<0.001. Both types of PFFs led to a decrease of viability of approximately 25% independent of sonication. Dopamine-stabilized oligomers led to the strongest decrease of viability.
Coomassie gel stain of human dopamine-stabilized alpha synuclein oligomers (SPR-466). Load: 2 ug.