Skip to content

Basket

You currently have no items in your basket.

Total (excl. vat) £0.00
View basket & checkout
Proteins and Peptides

Tau-441 (2N4R) Wild-Type Monomers (Baculovirus/Sf9)

Product Sizes
100 ug
SPR-496-100UG
2 x 100 ug
SPR-496-2X100UG
5 x 100 ug
SPR-496-5X100UG
About this Product
SKU:
SPR-496
Additional Names:
MAPT, intracellular neurofibrillary tangles, NFTs, paired helical filaments, PHFs, 2N4R
Application:
Cell-based/Functional Assay, SDS-PAGE, Western Blot
CE/IVD:
RUO
Concentration:
2 mg/ml
Extra Details:
Brain-specific tau isoforms vary in the number of N-terminal inserts and C- terminal repeat domains due to alternative splicing of exons; the 2N4R isoform of tau is expressed in adult brain yet is absent from the fetal brain (1). Tau (tubulin-associated unit) is normally located in the axons of neurons where it stabilizes microtubules. Tauopathies such as Alzheimer's Disease (AD) are characterized by neurofibrillary tangles containing hyper-phosphorylated tau fibrils (2). Hyper-phosphorylated tau can be generated via expression in the Sf9/Baculovirus system, with up to 20 sites confirmed by mass spectrometry and Western Blots with phospho-specific antibodies(3). Sf9/Baculovirus-expressed Tau 2N4R will readily form oligomers and fibrils in the absence of heparin.
Immunogen:
Tau-441 (2N4R) Wild-Type
Molecular Weight:
45.84 kDa
Purity:
>95%
Purification:
Ion Exchange
Sequence:
MAEPRQEFEVMEDHAGTYGLGDRKDQGGYTMHQDQEGDTDAGLKESPLQTPTEDGSEEPGSETSDAKSTPTAEDVTAPLVDEGAPGKQAAAQPHTEIPEGTTAEEAGIGDTPSLEDEAAGHVTQARMVSKSKDGTGSDDKKAKGADGKTKIATPRGAAPPGQKGQANATRIPAKTPPAPKTPPSSGEPPKSGDRSGYSSPGSPGTPGSRSRTPSLPTPPTREPKKVAVVRTPPKSPSSAKSRLQTAPVPMPDLKNVKSKIGSTENLKHQPGGGKVQIINKKLDLSNVQSKCGSKDNIKHVPGGGSVQIVYKPVDLSKVTSKCGSLGNIHHKPGGGQVEVKSEKLDFKDRVQSKIGSLDNITHVPGGGNKKIETHKLTFRENAKAKTDHGAEIVYKSPVVSGDTSPRHLSNVSSTGSIDMVDSPQLATLADEVSASLAKQGL
Shipping Conditions:
Dry Ice
Storage Conditions:
-70[o]C
Supplier:
StressMarq Biosciences
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Recombinant Proteins
SDS-PAGE of purified Sf9/Baculovirus-expressed hTau wild-type 2N4R on a 12% Tris-Glycine Gel. Lane 1: Biorad All Blue Standards (3uL). Lane 2: Baculovirus expressed hTau 2N4R Wild-Type (2ug). Lane 3: Lane 2: Baculovirus expressed hTau 2N4R Wild-Type (4ug).
Dot Blot of purified hTau wild-type 2N4R using phospho-specific Tau antibodies comparing E.coli-expressed material to Sf9/Baculovirus-expressed material. Protein was blotted on nitrocellulose, incubated with 1:1000 primary antibody and/or 1:4000 secondary antibodies. Exposed 0.6s second.
Modified/Total phosphorylation PTM spectrum counts reveal up to 20 phosphorylation sites on human wild-type Tau 2N4R monomers expressed using Baculovirus/Sf9 as determined by mass spectrometry. Protein sequence coverage was 76%. Localization probability cutoff set at ≥80% (yellow) or ≥95% (green).