Human Periphery Blood Mononuclear Cells and Isolated Immune Cells from PBMCs
2BScientific Limited have collaborated with IQBiosciences to establish high quality PBMCs from donors which are isolated for careful processing of immune cell types.
Along with Normal high-quality donor PBMCs we are also able to provide PBMCs that are irradiated and are from Non – Small Cell Lung Cancer donors and from Chronic Lymphocytic leukaemia blood donors sourced from renowned blood centres
The whole blood comprises of Plasma, mixture of cells and proteins. These components are required for blood to clot, circulation of oxygen in RBCs and to help fight infections through mononuclear cells which are also called PBMCs.
The PBMCs are crucial in research studies as they can be further isolated and characterised into a population of myeloid and lymphoid cells.
The myeloid cells include monocytes, dendritic cells, and macrophage that circulate through the blood to different tissues and engulf and destroy foreign cells in their immature state. They help with innate immunity and in their mature state can present the antigens to T cells thereby being called Antigen Presenting Cells.
The lymphoid cells consist of B cells, NK cells and T cells that are responsible for the antibody mediated adaptive immune responses by creating memory T and B cells.
The most common method for PBMC isolation involves using a density gradient medium (e.g., Ficoll-Paque or Lymphoprep).
- The density gradient medium separates different blood components, with PBMCs forming a distinct layer between the plasma and Red Blood Cells
These PBMCs can be further isolated by stimulating the cells with specific culture mediums, magnetic beads, and cell activators to accomplish T, B and NK cells generation.