Carrying out protein expression without needing a living host cell creates many advantages, which have been listed below.

Protein expression rates/Solubility

The CellFree system has higher expression rates and solubility than other protein expression systems. Using CellFree Sciences products and services may resolve issues with proteins that could not be expressed using other expression systems, or became insoluble after expression.
In most cases, the highest yields can be obtained through the wheat germ cell free expression system.

 Reproducibility/Scalability

The amounts of protein expressed are more or less constant no matter how many times the expression is carried out, as the system has a high reproducibility.

Additional syntheses and cost calculations can be done easily once the target protein yield is confirmed, which helps to efficiently plan ahead.

 Ease of Operations

Proteins can be obtained using only a pipette to adjust the reagents, and an incubator to control temperature.

No Codon Optimisation Necessary

A wheat germ cell-free protein expression system is less sensitive to codon bias , so there is no need for codon optimization (no preferred codon changes are needed for the host), and the genes from the original species can be used as is.

The is no decrease in the amounts expressed, even for mRNA with a unique sequence.

Selectivity of synthesis conditions

The conditions needed for protein expression are flexible, as the RNase activity for the wheat germ extract is extremely low and the reaction conditions for the translation stage can be changed freely depending on the purpose.
Variables such as temperature, time and reactivities can all be changed depending on what is desired.

Can be used internationally

There are no limitations to obtaining proteins from these systems internationally, as they do not fall under the Act on the Conservation and Sustainable Use of Biological Diversity through Regulations on the Use of Living Modified Organisms.
This allows for international group projects to use the CellFree system without the hassle of export restrictions.