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FLEXIQuant Protein Expression Kit

Wheat Germ Cell-Free Expression System to Make Full-Length Isotope-Labeled MS Standards

Advantages

  • Premixed reagents for easy reaction setup using high performance wheat germ extract WEPRO®9240 MS
  • WEPRO®9240 MS includes [13C, 15N]-L-Lys and [13C, 15N]-L-Arg suitable for working with trypsin digestion
  • Very high labeling efficiency with incorporation rates of ~99%
  • FLEXIQuant vector provided to utilize FLEX-tag for absolute protein quantification
  • Option to use His-tag-mediated purification for protein MS standard
  • Use FLEX-tag and FLEX peptide standards for two-step quantification of target proteins

FlexiQuant

Technical Details

  • Prepare expression vector for FLEX-tagged protein of interest using vector provided with kit
  • Prepare isotope-labeled FLEX-tagged MS Standard using reagents provided with kit
  • Use Western Blotting to determine concentration range of MS Standard and target protein in sample
  • Add MS Standard directly sample before processing
  • To copurify and digest MS Standard together with proteins in extract
  • Add FLEX peptide provided with kit before peptide analysis by MS
  • Determine protein concentrations using protein MS Standard and FLEX peptide
  • Detailed protocol in the literature: A practical guide to the FLEXIQuant method. Singh S, Kirchner M, Steen JA, Steen H. Methods Mol Biol. 2012;893:295-319.

FlexiQuant1

Ordering Information

SKUProduct NameDescriptionReactions
CFS-FLEX-MSFLEXIQuant Protein Expression Kit – MS Standards*Protein synthesis kit with 13C/15N labeled lysine and arginine for MRM/SRM Mass Spectrometry8 rxns
Kit provides vector EU-E01-TEV-N1-FLEX-tag
Kit provides FLEX peptide
Kit uses bilayer reaction format per reaction format on 226 μl scale
One reaction can produce ~23 μg of crude CDC27 protein (positive control included in kit)
EDX-FLEX-PEPFLEX-tag PeptideLyophilized, synthetic FLEX-tag peptide (TENLYFQGDISR); > 95% peptide purity, 1 nmol dried peptide per vial8 vials
Related Products
EDX-PLUS-MSPremium PLUS Expression Kit for MSProtein synthesis kit with 13C/15N labeled lysine and arginine for MRM/SRM Mass Spectrometry16 reactions
Kit uses bilayer reaction format on 226 μl scale
One reaction can produce ~30 μg of crude DHFR protein (positive control included in kit)
  • CFS can provide all our expression vectors free of charge
  • Inquire about our protein expression services: CFS can prepare 13C/15N lysine/arginine labeled proteins for MRM/SRM Mass Spectrometry
  • Request reference list with examples on accurate quantitative analysis by FLEXIQuant methods